The official journal of The Sri Lanka Veterinary Association
Sri Lanka Veterinary Journal (volume: 51(2))
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Volume - 51(2)
Year - 2004
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Section - A
Original Article
F. Noordeen1,  R.P.V.J. Rajapakse2,  N.U. Horadagoda2,  A.C.M. Faizal3  and  S. Appuhamy3
1. Department of Microbiology, Faculty of Medicine, University of Peradeniya
2. Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya
3. Veterinary Research Institute, Peradeniya

IDENTIFICATION OF CRYPTOSPORIDIUM PARVUM IN GOATS AND ITS IMPLICATION TO HUMAN CRYPTOSPORIDIOSIS

 F. Noordeen1 B.V.Sc., M.Phil., R.P.V.J. Rajapakse2 B.V.Sc., Ph.D., N.U. Horadagoda2 B.V.Sc., Ph.D., A.C.M. Faizal3 B.V.Sc., Ph.D. and S. Appuhamy3 B.V.Sc., Ph.D. 

  1. Department of Microbiology, Faculty of Medicine, University of Peradeniya
  2. Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya
  3. Veterinary Research Institute, Peradeniya

 

The present study was undertaken to determine the zoonotic potential of the Cryptosporidium species isolated from goats in Sri Lanka.  Thirty Cryptosporidium positive faecal samples were collected from goats in the wet, intermediate and dry zones of the country (n=10/zone).  The samples were subjected to microscopical examination following a modified Zihel Neelsen staining.  DNA extracted from these samples was used for Polymerase Chain Reaction (PCR); 15 isolates (dry zone, 8; intermediate zone, 7) were identified as Cryptosporidium parvum.  Five randomly selected isolated were identified as Cryptosporidium parvum genotype 2.  The isolation of Cryptosporidium parvum genotype 2 in goats suggests that the organism is of potential zoonotic importance to Sri Lanka.

S.L.Vet.J. 2004, 51(2A): 1-4

Clinical Communication
D.R.A. Dissanayake1,  T.G. Wijewardana1  and  G.K.M.C.Ranasinghe2
1. Department of Veterinary Pathobiology, Faculty of Veterinary Medicine & Animal Science, University of Peradeniya
2. Department of Farm Animal Production & Health, Faculty of Veterinary Medicine & Animal Science, University of Peradeniya

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Short Communication
W.R.A.K.J.S. Rajapaksha1,  T.P. Wijesekera2,  I.D.S.I.P. Thilakaratne1  and  A.D.N. Chandrasiri1
1. Veterinary Research Institute, Gannoruwa, Peradeniya
2. Postgraduate Institute of Agriculture, University of Peradeniya

DEVELOPMENT OF PCR ASSAY FOR THE IDENTIFICATION OF DOG MEAT

W.R.A.K.J.S. Rajapaksha1 B.V.Sc., Ph.D., T.P. Wijesekera2 B.Sc., I.D.S.I.P. Thilakaratne1, B.V.Sc., A.D.N. Chandrasiri1 B.V.Sc., Ph.D. and T.D. Niroshan1 B.V.Sc.

1.      Veterinary Research Institute, Gannoruwa, Peradeniya

2.      Postgraduate Institute of Agriculture, University of Peradeniya

A polymerase chain reaction (PCR) assay was developed to differentiate dog meat from the meat of Ceylon spotted deer (Axis axis ceylonensis), Ceylon sambhur (Cervus unicolor unicolor), cattle, goat, buffalo and sheep.  A set of primers were designed according to the sequence of the mitochondrial cytochrome b gene of canis familiaris and by PCR amplification 376 bp band was observed only for dog breeds and these primers did not cross-reacted with DNA of other animal species studied.  A band of 649 bp size was observed for all animal species when DNA was amplified with the universal primers and that indicated the presence of mitochondrial DNA in the samples.  The absence of cross reaction with human DNA, the dog specific primers eliminates the possible false positive reaction.

S.L.Vet.J. 2004, 51(2A): 9-11

Section - B
News

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Prof. S. T. Fernando Memorial Lecture
D.J. Weilgama1
1. Department of Parasitology, Faculty of Medicine, University of Peradeniya

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Abstracts

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