The official journal of The Sri Lanka Veterinary Association
Sri Lanka Veterinary Journal (volume: 60(1))
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Volume - 60(1)
Year - 2013
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Section - A
Review Article
B.V.P. Perera1
1. Department of Wildlife Conservation, Sri Lanka

Abstract is not available for this review article. Please click on Read button to read  online or Download button to download the PDF of this review article.

Original Article
G.D.R.K. Perera1,  P.G.A. Pushpakumara1,  L.N.A.de Silva1  and  Basil Alexander1
1. Animal Embryo Biotechnology Laboratory, Department of Farm Animal Production and Health, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka

PRELIMINARY STUDY ON FREEZING (VITRIFICATION) OF IN VIVO DERIVED EMBRYOS AND THEIR
TRANSFER IN GOATS IN SRI LANKA

G.D.R.K. Perera, P.G.A. Pushpakumara, L.N.A.de Silva and Basil Alexander

Animal Embryo Biotechnology Laboratory, Department of Farm Animal Production and Health, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka

Embryo biotechnologies are innovative studies in Sri Lanka. Embryo freezing is a process that
can be used to preserve embryos for future embryo transfer programs. The objective of the study was to
validate goat embryo freezing technology using ethylene glycol and glycerol under field situation in Sri
Lanka.
In the present study 10 embryo donors were super ovulated under field situation with pure ovine FSH
(oFSH) hormone. They were kept for 48 h with two bucks for breeding. Embryos were collected 7 days after
estrus, by flushing of the uterus after laparotomy. Selected good quality embryos were divided into two
groups. Group 1 embryos (n=7) were frozen with Ethylene Glycol while group 2 (n= 8) with Glycerol.
Embryos in each group were transferred and kept in the relevant freezing medium for 5 minutes for
equilibrium and each embryo was loaded into 0.25 cc straws. The straws were frozen and stored in liquid
nitrogen. Six months later, straws were thawed at 370C for 30 seconds and six embryos in each group were
transferred into estrous synchronized recipients (n=6) 7 days post-estrus.
Six does responded to the superovulatory treatments yielding 24 embryos. The embryo recovery rate was
83%. Fifteen embryos were selected for freezing while 9 were rejected due to poor quality. One embryo in
each group was lost while another one in group 2 was damaged during the freezing and thawing process.
Following transplantation, one doe was found to be pregnant in Group 1. Five months later a healthy female
offspring was born with 2.4 kg birth weight. During the first six weeks weight gain of the kid was 149.6g /day.
This study highlighted that it is feasible to freeze in-vivo produced goat embryos under field condition
with ethylene glycol.

S.L.Vet.J. 2013, 60(A): 13-17

Short Communication
D.K.Ekanayake1  and  R.P.V.J Rajapakse1
1. Department of Pathobiology, Faculty of Veterinary Medicine and Animal science, University of Peradeniya, Peradeniya, Sri Lanka

OCCURENCE AND DISTRIBUTION OF GASTROINTESTINAL PARASITES OF THE LOCAL VILLAGE CHICKENS (GALLUS GALLUS DOMESTICUS) IN THREE CLIMATIC ZONES OF SRI LANKA

D.K.Ekanayake and R.P.V.J Rajapakse

Department of Pathobiology, Faculty of Veterinary Medicine and Animal science, University of Peradeniya, Peradeniya, Sri Lanka

Occurrence and distribution of gastrointestinal parasites in 43 free ranging village hens and 57
cockerels (Gallus galus domesticus) in 7 district representing 3 climatic zones of Sri Lanka were studied.
Twenty eight birds were from wet zone, 51 birds and 21 birds were from intermediate and dry zone area
respectively.
Parasites were found in 90% birds, 84% had helminths and 53% had Eimeria spp. The prevalence of
helminth species varied; Railleitina tetragona (57%), Heterakis gallinarum (45%), Ascaridia galli (21%),
Raillitina echinobothrida (22%), Hymenolepsis species (17%) and Capillaria (13%). Similarly mean counts
of different helminth species also varied (mean±SE); H.gallinarum (9±2.7), A.galli (0.94±0.31), E. tetragona
(1.84±0.21), R. echinobothrida (0.42±0.1) and Hymenolepsis spp (0.3±0.07). There was no significant
difference observed in the occurrence of both Eimeria species and helminthiasis in different climatic zones
(P>0.05). This study can be used as a useful guide on the occurence of parasites in free ranging village
chickens along with its local distribution around three climatic zones of Sri Lanka.

S.L.Vet.J. 2013, 60(A): 19-21

Section - B
News

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Review Article
D.H.A.Subasinghe1  and  H.Abeygunawardena1
1. Faculty of Veterinary Medicine and Animal Sciences, University of Peradeniya

Abstract is not available for this review article. Please click on Read button to read  online or Download button to download the PDF of this review article.