The official journal of The Sri Lanka Veterinary Association
Sri Lanka Veterinary Journal (volume: 62)
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Volume - 62
Year - 2015
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Section - A
Original Article
Basil Alexander1
1. Department of Farm Animal Production and Health, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka

SUMMARY: The aim of the study was to investigate the microtubules and chromatin dynamics during the first cell cycle of sheep clone embryos activated with either 6-dimethylaminopurine (6-DMAP) or cycloheximede (CHX). Fetal fibroblasts were transplanted into enucleated oocytes. The reconstructed cell-cytoplast couplets were fused and activated using ionomycin followed by culture in two separate groups containing 6-DMAP (2mM) or CHX (10 mg/ml) for 3 hours. At one hour post-fusion, 63.3% of reconstructed embryos underwent nuclear envelope break down (NEBD) and premature chromosome condensation (PCC). At one hour post-activation (hpa), a clearly visible pronucleus (diameter 6-8 μm) had already formed in the embryos treated with 6-DMAP but not in the CHX-treated group. At three hours post-activation, the 6-DMAP and CHX-treated embryos showed one swelled pronucleus with a mean diameter of 8.4 ± 1.3 μm and 25.8 ± 0.8 μm, respectively (P<0.05). In the 6-DMAP-treated embryos, most of the embryos showed a metaphase spindle as early as 16hpa but not in CHX-treated group. Typical 2 cell division was seen in most of the 6-DMAP treated embryos 28 hpa, and it was slightly delayed in the CHXtreated embryos. In summary, the artificial activating agents, 6-DMAP and CHX, exhibited different effects on chromatin remodeling, cell cycle progression rate and on the degree of pronuclear swelling which may explain the poor developmental rates and abnormal chromosome complements observed in the cloned embryos.

Short Communication
Rucha Borkar1,  Kabita Roy2,  P.C. Shukla1,  Amita Tiwari1  and  I.C. Datta1
1. Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, The Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP- 482 001, India
2. Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Madhya Pradesh Pashu Chikitsa Vigyan Vishwa Vidyalaya, Jabalpur-482 001 MP, India

SUMMARY: A study was conducted on client-owned dog patients brought for treatment in the Out Patient Department (Medicine), Teaching Veterinary Clinical Complex, Veterinary College, Jabalpur, M.P., India. Of the total 240 dogs with clinical signs of dermatitis, Malassezia dermatitis was confirmed in 60 cases by microscopic examination of the modified Wright stained impression smear and fungal culture on Sabouraud's dextrose agar plate. Synthetic antifungal, ketoconazole and flucoconazole were offered in two different doses (pulse therapy, 4 cycles). Herbal Neem (Azadarichta indica, Linn.) leaf extract powder at two graded dose levels was given with food daily for 4 weeks. Broad spectrum antibacterial, cephalexin for 5 days daily, and herbal hepatoprotective sylimarin, were offered orally daily in all six combination regimens. Herbal Neem seed oil (NSO) was applied topically on the skin lesions daily, and shampoo (2% miconazole plus 2% chlorhexidine), followed by body wash once a week was recommended. In all six treatment groups, the circulatory protein profile remained virtually unaltered. However, statistically significant (P <0.05) changes were recorded in Alanine aminotransferase with biomedical implications.

Clinical Communication
M.G.C.M Jayasinghe1,  K.G Indika1,  M.A.Y Chaturangani1,  A.M.R Bandara1,  A.M.S.M.B Wickramarathna1,  A. Dangolla1  and  D.D.N de Silva1
1. Veterinary Teaching Hospital, Department of Veterinary Clinical Sciences, University of Peradeniya

SUMMARY: The clinical presentations, diagnosis, treatment and management of seven free ranging black turtles (Melanochelis trijuga thermalis) presented to the Veterinary Teaching Hospital (VTH) University of Peradeniya, Sri Lanka during the period of 16 months (October 2013 to February 2015) are described. Clinical complications included shell fractures (n=4), fish hook lodged in the maxilla (n=1), avulsion of fore leg (n=1) and a minor abrasion on the dorsum (n=1). All were stabilized with warm intraceolomic (ICe) fluids (Normal Saline) before and during anaesthesia. All but one turtle were anaesthetized with ketamine (20-80 mg/kg) IM or IV, and propofol (5-10mg/kg, IV) was used in one turtle. Enrofloxacin (5-10mg/kg,IM) was used in all as an antibiotic and the pre- and post-surgical pain management was achieved with meloxicam (0.2mg/kg, IM). Orthopaedic wires were used for shell fracture stabilization while Plaster of Paris was used in one turtle in addition to orthopaedic wires. Fresh fractures were repaired after wound cleaning on the day of presentation and fracture management was delayed in one turtle with infected fracture (n=1). Enteral feeding through an esophagostomy tube, provision of water and adequate sunlight in order to maintain the body temperature were important considerations during the hospitalization period. The fish hook lodged in the maxilla was removed with a small pair of hemostat forceps after opening the mouth using a tongue depressor, laceration due to the penetrated hook was sutured with absorbable suture materials. The right fore leg was amputated from the shoulder joint in the turtle with the avulsion of fore leg. Five out of seven turtles recovered after the hospitalization, and four turtles were released to their natural habitat while one turtle is maintained in captivity.

Section - B
News

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Reports
D.H.A.Subasinghe1
1. Faculty of Veterinary Medicine and Animal Sciences, University of Peradeniya

Abstract is not available for this review article. Please click on Read Full Text to access this review article.