The official journal of The Sri Lanka Veterinary Association
Sri Lanka Veterinary Journal (volume: 63 (1))
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Volume - 63 (1)
Year - 2016
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Section - A
Original Article
D.R. Anuruddhika Dissanayake1  and  T.G. Wijewardena1
1. Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka

SUMMARY: Avian pathogenic Escherichia coli (APEC) cause a wide range of economically significant infections in chickens. Control of these infections by antimicrobial drug is no longer possible due to high prevalence of multidrug resistance strains. Involvement of large number of serotypes in these infections left none serotype specific vaccine as the only option. It has been well established that the Lipopolysaccharide (LP) core specific antibodies are broadly cross protective. Present study determined the LPS core specific antibody titers of chickens immunized with a single dose of heat killed rough mutant E. coli strains comprising of LPS core types R1, R2, R3 and R4. Mean anti-LPS core antibody titres for all four core types increased significantly (p<0.05) in immunized group than in control group. Thus, the heat killed mixture of rough mutant E. coli strains can be used as a vaccine to enhance LPS core specific antibodies in chickens.

Short Communication
G.D.B.N.Kulasooriya1,  P.P.Jayasekara1,  J.M.S.M.Wijayarathna1,  M.K.U.T.Amarasiri1,  B.C.G.Mendis2,  A.Siribaddana3,  A.Dangolla4,  R.S.Kalupahana1  and  B.R.Fernando1
1. Department of Veterinary Public Health and Pharmacology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya
2. Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya
3. Respiratory Unit, Teaching Hospital Kandy
4. Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya

SUMMARY: During religious, cultural and other human entertainment activities domesticated elephants come into close contact with humans creating a potential disease transmission threat between elephants and humans. The main objective of the study was to screen the elephants participated in the Esala Perahera 2015 for the zoonotic bacterial pathogens namely Salmonella, Campylobacter and Mycobacterium tuberculosis complex. Further, the antimicrobial susceptibility of the fecal E. coli and zoonotic pathogens isolated were determined to identify multidrug resistant organisms. The isolation rates for E.coli and Salmonella were 100% and 8%, respectively while Campylobacter was not isolated from any of the fecal samples collected. Three of the four Salmonella isolates were resistant to ampicillin and tetracycline and all four isolates were susceptible to nalidixic acid, streptomycin, gentamicin, ciprofloxacin, sulfamethoxazole and trimethoprim combination, cefotaxime, ceftazidime, ceftriaxone, imipenem and amikacin. A number of E.coli isolates were resistant to ampicillin (8%), sulfamethoxazole and trimethoprim combination (8%), tetracycline (8%), ceftriaxone (8%), amikacin (6%), nalidixic acid (4%), imipenem (4%), gentamicin (2%), streptomycin (2%), ceftazidime (2%) and ciprofloxacin (2%). However, all tested E.coli isolates were susceptible to cefotaxime. Further, 8% of the E. coli isolates showed resistance to three or more antimicrobial groups used and can be classified as multidrug resistant. None of the elephants yielded a positive result for the fecal PCR assay indicating that the animals did not excrete pathogenic mycobacteria in their feces.

Review Article
W.R. Bandula Kumara1
1. Veterinary Teaching Hospital, Department of Veterinary Clinical Science, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka

SUMMARY: Canine babesiosis is an important tick-borne disease caused by hemoprotozoan parasites of the genus Babesia, and the predominant species infecting dogs is Babesia gibsoni. The disease has been identified worldwide and is now recognized as a serious emergent disease in dogs. Although the incidence of canine babesiosis has been increasing, effective and crucial therapeutic modality is unavailable. The conventional antibabesial drugs including diminazene aceturate, pentamidine, and imidocarb dipropionate could not eliminate the parasites from infected dogs. Newer therapeutic modality for canine babesiosis using drugs such as clindamycin, atovaquone and multiple drug combinations has been suggested. Although these therapeutic modalities are beneficial and seem to partially provide a permanent cure, the treatments failures often occur. Thus, broad understanding of treatment protocols are required when implementing an appropriate treatment plan against canine babesiosis. The goal of this review is to provide veterinary practitioners with guidelines for successful treatment against canine babesiosis. It is expected that the review will answer the most frequently asked questions posed by veterinary practitioners.

Clinical Communication
K.G. Indika1,  M.P. Kumara1,  H.M.H.S. Ariyarathna1,  M.G.C.M. Jayasinghe1,  A.M.R.B. Adikari1  and  D.D.N de Siva1
1. Department of Veterinary clinical Science, University of Peradeniya

Summary: Liposarcomas are rare malignant tumors of adipose tissues with overall incidence of 0.2–0.5% among all the canine neoplasms. They may originate either from bone marrow or extra skeletal sites such as adipose tissues. Histological classification include well differentiated, pleomorphic, and myxoid subtypes. Clinical cytological, gross pathological and histopathological features of two extra skeletal canine liposarcoma cases presented to the Veterinary
Teaching Hospital (VTH), University of Peradeniya are described here.

D.R.A. Dissanayake1,  I. D. Silva1,  Y.U. de Silva Senapathi1,  D.D.N. de Silva1,  M.D.H.S. Mallikarachchi1,  W.G.D.A. Gunathilaka1,  W.R.B Kumara1,  E. Rajapaksha1  and  W.C.R. Fernando1
1. Veterinary Teaching Hospital, Department of Veterinary Clinical Science, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya

Summary: Two adult dogs aged 3.5 and 9 years were diagnosed with acquired Myasthenia Gravis (MG) based on clinical signs and positive response to oral neostigmine bromide (0.25mg/Kg BW) within 4 hours of oral administration. Both patients were successfully treated using neostigmine (1mg/Kg BW daily) alone or with neostigmine (1mg/Kg BW daily) and prednisolone (1mg/Kg BW, q12h) combination. Serum alkaline phosphatase and albumin/ globulin ratio monitored in one of the above patients starting from one month prior to development of generalized clinical signs until complete recovery, showed significant alterations which can be used in diagnosing MG and in monitoring the response to treatment.

Section - B
Technical Note
Indira D. Silva1
1. Department of Veterinary Clinical Sciences, University of Peradeniya

Summary: Not avialable for this technical note. Please download the fulltext for details.

Reports
D.H.A.Subasinghe1
1. Faculty of Veterinary Medicine and Animal Sciences, University of Peradeniya

Summary: Not available for this report. Please download the fulltext for details.

News

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