The official journal of The Sri Lanka Veterinary Association
Sri Lanka Veterinary Journal (volume: 64(2))
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Volume - 64(2)
Year - 2017
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Original Article
M. G. Thammitiyagodage1,   P. Somaratne1,  M.A. Roshan Priyantha2  and  K.C.R. Perera1
1. Medical Research Institute, No. 527, Dr Danister de Silva Mawatha, Colombo 8
2. Veterinary Research Institute, P.O. Box 28, Gannoruwa, Peradeniya

Leptospirosis is an important zoonotic disease in man and animals. Increasing numbers of human Leptospirosis are reported each year in Sri Lanka. Rodents are most often the natural maintenance host of leptospira, but the potential of transmitting the disease from ruminants and dogs to human should not be overlooked. Aim of this study was to determine the serum antibody titres for leptospira in 139 dogs in selected suburbs of Colombo, Sri Lanka and evaluate its association with dog rearing pattern and immunization history. A questionnaire based survey was conducted to collect data on dog rearing pattern and vaccination history of dogs. In addition, the potential transmission of leptospira from dog to human and public awareness on canine leptospirosis was also assessed. Blood samples obtained from dogs were subjected to Microscopic Agglutination Test (MAT) to detect leptospira antibody levels and to identify respective strains. Strains were identified against 12 serovars of pathogenic genomospecies Leptospira interrogans. According to MAT results, around 62% of unvaccinated domestic dogs enrolled in the study had leptospira antibody titres ranging from 1:100 - 1:3200, indicating an acute or convalescent stage of infection. Moreover, 25% of primarily vaccinated dogs, 17.9% of regularly vaccinated dogs and 36.8% of randomly vaccinated dogs failed to seroconvert. Serum samples subjected to sero grouping were all found to be within pathogenic genomic species Leptospira interrogans. Single sero groups L. canicola, L. australis, L. Icterohaemorragica and L. djsiman and mixed sero-groups L. australis and L. canicola were predominantly observed. No clinically proven Leptospirosis was reported among the owners of the dogs, their family members or other household pets even if they had close contact with their dogs. Among the surveyed population, only one person was aware of (0.7%) possible transmission of leptospirosis from dogs. Statistically significant number of primarily vaccinated dogs was in indoor habitats and randomly vaccinated dogs were in outdoor habitats (p< 0.05). That trend was not observed in regularly vaccinated and unvaccinated dog population (p> 0.05). Leptospirosis being a highly infectious zoonotic disease, outdoor living unvaccinated and randomly vaccinated domestic canine populations may contribute to increasing incidence of Leptospirosis in man as well as in other domestic mammals.

M.W.D.C. Weerathunga1,  J.K.H. Ubeyratne2  and  M.A.Nadheer1
1. Department of Animal Production and Health, Ampara, Sri Lanka
2. Veterinary Research Institute, Gannoruwa, Peradeniya, Sri Lanka

Haemorrhagic septicaemia (HS) is a major diseases of bovines in Sri Lanka before 2005. The disease is endemic in dry zone where clinical incidences occur with the onset of monsoon. An outbreak of HS occurred in twenty 1-3 year old local buffaloes of which 15 died, in mid-October 2016 in Navithanveli veterinary range in Ampara District. The disease continued and another 13 buffaloes and 3 cattle died. Clinical and necropsy findings and laboratory identification confirmed that the causative organism was Pasrerella multocida serotype B:2. Outbreak was controlled with treatment and vaccination of all susceptible animals. This is the first report of the re-emergence of P. multocida after 2000 in Ampara district.

Indira Silva1,  K. Thananjayan1,  DRA Dissanayake1,  WCR Fernando2  and  M. Murugananthan3
1. Veterinary Teaching Hospital, University of Peradeniya, Sri Lanka
2. Department of Animal Production and Health, Sri Lanka
3. Department of Parasitology, Faculty of Medicine, University of Jaffna, Sri Lanka

Assessment of erythrocyte morphology is an important aid in diagnostic haematology. Anisocytosis which is changes in RBC size, correspond to changes in diameter and surface area (SA) of the cell, and are not always reflected in the Mean Corpuscular volume (MCV). This paper discusses the relationship of MCV with surface area and diameters (vertical and horizontal) in 2D views of erythrocytes of clinically healthy dogs using confocal microscopy. This information would be valuable for early detection of cellular changes in dogs in subclinical or clinical diseases.

The average diameters, average SA, and MCV values of RBCs studied were in normal distribution and the mean values of 7.169 ± 0.648 μm for horizontal diameter (D1), 7.1245 ± 0.6646 μm for vertical diameter (D2), and 41.061 μm2 ± 6.866 for SA did not reveal a significant correlation or a strength of association with the MCV values, indicating that the MCV value has limitations as an objective measurement of detecting anisocytosis.

R.M.S. Pimburage1,  P.A.L. Harischandra1,  M. Gunatilake2,  D.N. Jayasinhe1,  A. Balasuriya3  and  R.M.S.K. Amunugama4
1. Public Health Veterinary Services, Ministry of Health, Sri Lanka
2. Department of Physiology, Faculty of Medicine, University of Colombo, Sri Lanka
3. Faculty of Medicine, General Sir John Kotelawala Defence University, Sri Lanka
4. Ministry of Health, Sri Lanka

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